Immunofluorescence analysis is a technique used for light microscopy with a fluorescent microscope. This analysis mainly uses the specificity of antibodies to their specific protein to target fluorescent dyes and show the specific part of the cell that is desiredSDS-PAGE is a very commonly used technique used in the lab for the separation of proteins based on their molecular weight. SDS-PAGE separates the proteins based on their rates of migration through a gel under the influence of electricity.

 

In this article, an immunofluorescence analysis was done on the cell death inducing factors by using an antibody that corresponds to the inducing factors as well as a secondary antibody that gave off red fluorescence. While the cell death inducing factors were dyed with a red fluorescence, the nuclei of the cells were dyed with a blue fluorescence. The cells were later put under a microscope and photographed.