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Cancer-terminating virus containing MDA-7 inhibits neuroblastoma cell growth Cancer-terminating virus containing MDA-7 starts programmed cell death in neuroblastoma Cancer-terminating virus containing MDA-7 uses pathways that are different than normal cancer killing pathways Cancer-terminating virus containing MDA-7 promotes programmed cell death in neuroblastoma Cancer-terminating virus containing MDA-7 is increased by upstream molecules Cancer-terminating virus containing MDA-7 works in animals that have grown neuroblastoma tumors
Viral Construction Differentiation between Cancer and Non-cancer
Western Blot TUNEL Assay FACS Analysis SDS-PAGE Immunofluorescence Analysis MTT Assay

Journal of Comprehensible Results

P Bhoopathi, N Lee, A Pradhan, X Shen, S Das, D Sarkar, L Emdad, P Fisher (2016)
mda-7/IL-24 Induces Cell Death in Neuroblastoma
through a Novel Mechanism Involving AIF and ATM
Cancer Research, June 2016 76:12

(Translated by Krishna Karamsetty)

Abstract

Advanced stages of neuroblastoma, the most common form of a tumor outside the brain in children under the age of 5 is resistant to therapy. The expression of melanoma differentiation-associated gene-7 or MDA-7 [1] promoted antitumor activity in petri dishes, animal models and in a clinical trial in patients with advanced cancers without harming normal, healthy cells. When a cancer has gone into its advanced stages, MDA-7 is suppressed therefore the forced expression of MDA-7 decreased tumor activity in many different forms of cancer [1].
 
In order to more effectively express MDA-7, a genetically modified virus or a cancer-terminating virus was created which replicated itself in cancer cells as well as begin the cell death process by injecting the cancer cells with the desired gene [2]. Most programmed cell deaths have a specific process that is followed, however in these experiments, MDA-7 has followed a completely different process in both live subjects and in petri dishes.
 
 

References

  1. Jiang H, Lin JJ, Su ZZ, Goldstein NI, Fisher PB. Subtraction hybridization identifies a novel melanoma differentiation associated gene, mda-7, modulated during human melanoma differentiation, growth and progression. Oncogene 1995;11:2477–86.
  2. Sarkar D, Su ZZ, Vozhilla N, Park ES, Gupta P, Fisher PB. Dual cancer-specific targeting strategy cures primary and distant breast carcinomas in nude mice. Proc Natl Acad Sci U S A 2005;102:14034–9.