Journal of Comprehensible Results

Hires SA, Zhu Y, Tsien R (2008)
Optical measurement of synaptic glutamate spillover and reuptake
by linker optimized glutamate-sensitive fluorescent reporters
Proc Natl Acad Sci USA 105:4411-4416

(Translated by Jeff Elhai)

Support: Overexpression of protein using pRSETB

[Explanation of promoter(PT7), ribosome binding site (RBS), His-tag (His6), EK [???], Multiple cloning sites (MCS), transcriptional terminator (Term), rest of plasmid for replication and selection in E.coli.]

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