Journal of Comprehensible Results

Hires SA, Zhu Y, Tsien R (2008)
Optical measurement of synaptic glutamate spillover and reuptake
by linker optimized glutamate-sensitive fluorescent reporters
Proc Natl Acad Sci USA 105:4411-4416

(Translated by Jeff Elhai)

Experiment: Expression of sensor in living cells

[Brief explanation of transfection]

[Brief explanation of HEK293 cells]

[image]
[Cartoon of plasmid into cell, regrown to dendrite]
[Membrane bound response in HEK293 cells to known amount of glutamate (100 μM), using original sensor and optimized sensor.]

[Change log scale to linear. Change "0N0C" to "original", "8N5C" to "optimized"]