[Brief explanation of HEK293 cells]
Hires SA, Zhu Y, Tsien R (2008)
Optical measurement of synaptic glutamate spillover and reuptake
by linker optimized glutamate-sensitive fluorescent reporters
Proc Natl Acad Sci USA 105:4411-4416
Optical measurement of synaptic glutamate spillover and reuptake
by linker optimized glutamate-sensitive fluorescent reporters
Proc Natl Acad Sci USA 105:4411-4416
(Translated by Jeff Elhai)
Experiment: Expression of sensor in living cells
[Brief explanation of transfection]
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[image]
[Cartoon of plasmid into cell, regrown to dendrite] |
[Membrane bound response in HEK293 cells to known amount of glutamate (100 μM), using original sensor and optimized sensor.]
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[Change log scale to linear. Change "0N0C" to "original", "8N5C" to "optimized"] |