Rodionova IA, Zhang Z, Mehla J, Goodacre N, Babu M, Emili A, Uetz P, Saier MH Jr. (2017)
The phosphocarrier protein HPr of the bacterial phosphotransferase system globally
regulates energy metabolism by directly interacting with multiple enzymes in Escherichia coli
J Biol Chem. 2017 Aug 25;292(34):14250-14257
The phosphocarrier protein HPr of the bacterial phosphotransferase system globally
regulates energy metabolism by directly interacting with multiple enzymes in Escherichia coli
J Biol Chem. 2017 Aug 25;292(34):14250-14257
(Translated by Shravani Wadwekar)
Implications: Protein-Protein Interactions
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All four of these proteins are seen as potential targets HPr could have interacted with; the steady state kinetics measured that HPr has direct interactions with PykF, PfkB, and NagB by allosterically regulating them, while HPr-P had direct interactions witj Adk by allosterically inhibition. These protein-protein interactions were seen through a rise in actitivity in the presence of HPr or HPr-P versus the absence. All of these enzymes are activated by HPr or HPr-P, as it is always present when there is a rise in activity.
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Figure iii. Figure shows a protein-protein interaction (PPI) in the quaternary form of protein structure. Source: https://www.creative-proteomics.com/blog/index.php/brief-introduction-of-protein-protein-interaction-ppi/ |