Home Abstract Introduction
Real-time PCR Analysis of p21, p53, PDGF, and FGF Western Blot Analysis of p21, p53, PDGF, and FGF Antibody Array
PDGF -BB plays a critical role in presenting an antisenescence effect through the upregulation of FGF 2
Real-Time PCR Supplementary Figures Antibody Array Supplementary Figures

Journal of Comprehensible Results

Yun-Ui Bae et al. (2016)
Antisenescence effect of mouse embryonic stem cell conditioned medium
through a PDGF/FGF pathway.
FASEB Jounal: Official Publication of the Federation of American societies for Experimental Biology, 30(3). 1276-86.

(Translated by Daanish Fiaz)

Introduction

The first steps they took towards this article is determining what aspect they would like to approach their issue from. They first cultured and conditioned HDFs as well as stem cells respectively. Then they performed a real-time PCR and Antibody Array to analyze p21, p53 expression as well as several other molecules including PDGF -BB, FGF 2, PDGF -AA.

The main question they asked was what factors are involved in presenting antisenescence activity? How can we understand the specificity of those factors in within their respective pathways? How do we confirm the results for PDGF mediated anti senescence effects? How is FGF 2 involved in this PDGF -BB anti senescence activity? Is FGF 2 the ultimate signal effector of the pathway for anti senescence? How do antagonists sunitinib and SU5402 affect PDGF -BB and FGF 2 within this pathway? Is this seen in younger cells as well? Sunitinib and SU5402 were antagonists utilized to confirm their results within the pathway.

PDGF -BB and FGF 2 are significant molecules within the PDGF/FGF pathway. But in what manner? The PDGF -BB actually upregulates FGF 2 resulting in anti senescence activity within the pathway of the cell (1). Sunitinib and SU5402 both inhibited PDGF -BB while only SU5402 inhibited FGF 2. This lead to the conclusion that ultimate downstream effector protein in the PDGF mediated anti senescence effect (2). These tie back to the understanding of the anti senescence activity of these factors within the PDGF/FGF pathway. Aiding in the understanding of how the PDGF/FGF pathways is mediated by PDGF -BB thus presenting antisenescence activity within the cells conditioned.

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