Home Abstract Introduction
Cross-linking Cell Lysis and DNA Fragmentation Chromatin immunoprecipitation and elution of DNA cross-link reversal and DNA Purification preparation of DNA for Sequencing Next Generation Sequencing process
KLF1 peaks Distance of promoter peaks Analysis of KLF1 binding to gene body of ZBTB7A

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Laura J. Norton, Alister P.W. Funnell (2017)
KLF1 directly activates expression of the novel fetal globin repressor ZBTB7A/LRF in erythroid cells
Blood Advances 1:685-692

(Translated by Wassal Alhammad)

Implications: Analysis of KLF1 binding to gene body of ZBTB7A

We next performed ChIP sequencing (ChIP-Seq) experiments in both mouse cell lines to determine whether KLF1 binds directly to the ZBTB7A promoter.

In mouse contexts, we identified the ZBTB7A promoter as one of the most highly enriched KLF1 peaks.

This Figure shows KLF1 enrichment across the gene body of Zbtb7a. zooming in, KLF1 enrichment at the promoter region of Zbtb7a, showing the conserved CACCC and GATA consensus sequences in pink and gray, respectively

Fig. 14: KLF1 peaks are much larger at the promoter region of Zbtb7a .