Mouse ChIP-Seq experiments were performed in, KLF1 immortalized erythroid “K1ER” cells, which are preserved red blood cells. The distribution of KLF1 peaks across promoters, exons, introns, and other regions across the Mus musculus and Homo sapiens genomes were analyzed based on RefSeq annotations. In figures 11 and 12, Promoter regions were defined, intronic regions were those lying between exons, and intergenic regions made up the remainder of the genome. Peaks that fell into coding exons or 59 and 39 untranslated region exons or close to the transcription termination sites were labeled as other. Figure 11 shows a total of 50 888 KLF1 peaks for K1ER cells were found. a large number of KLF1 peaks fell within introns, with 43% in K1ER cells. An enrichment of peaks was also found at promoter regions, with 16% in K1ER cells. A large representation of intergenic regions was also seen, with 35% in K1ER cells.

In Figure 12, we included an analysis of the top 800 KLF1 peaks in K1ER -2 cells as a direct comparison. As seen in the pie chart, Promotor has the majority of KLF1 peaks. With 56% in K1ER cells.

Fig. 11: the all KLF1 peaks on the genome of ZBTB7A

Fig. 12: Top 800 of KLF1 peaks on the genome of ZBTB7A