Home Abstract Introduction
Cross-linking Cell Lysis and DNA Fragmentation Chromatin immunoprecipitation and elution of DNA cross-link reversal and DNA Purification preparation of DNA for Sequencing Next Generation Sequencing process
KLF1 peaks Distance of promoter peaks Analysis of KLF1 binding to gene body of ZBTB7A

Website for translation of

Laura J. Norton, Alister P.W. Funnell (2017)
KLF1 directly activates expression of the novel fetal globin repressor ZBTB7A/LRF in erythroid cells
Blood Advances 1:685-692

(Translated by Wassal Alhammad)

Experiment: Next Generation Sequencing process

After preparing DNA by adding adapters and amplifying it. The Adapter-Modified DNA can be sequenced by a machine called HiSeq 2500 (Illumina). This machine is high-throughput sequencing system that synthesize/read DNA by nucleotide.

After reading the sequence of the DNA fragments, The sequence analysis program will map the obtained sequences to the genome database and give a report on where the precipitated DNA are located.

The locations of the precipitated DNA indicate the location where KLF1 was binding in the genome when the cells are collected.

Fig. 9: HiSeq 2500 (Illumina) machine. From
illumina website.
 
Fig. 10: detailed DNA Report from the sequence analysis program. From
CoreGenomics website.