Therefore DNA sequencing start with tow preparation steps: Ligating/pasting Sequencing Adapters to DNA Fragments, then amplifying it by PCR. The Adapters are short fragments of DNA that can be ligated to any piece of DNA and can be used as templates for polymerase chain reactions and sequencing process.
Adapter-Modified DNA are amplified by PCR, which is a specific technique to get a good amount of identical copies of the Adapter-Modified DNA fragments. It is performed using a DNA polymerase, which is enzyme that responsible for synthesizing DNA molecules.
The amplified DNA is now ready for sequencing.