Study Guide III - MoAb's and Peptides
- Let's talk MoAbs - download the PPT
- How do they differ from peptide/amino acids
- Lock and key
- Receptor binding
- Define the Ab_Ag relationship
- Ag - Determinants/epitopes
- B lymphocytes - Plasma and Memory cells response
- Poly antibody - Polyclonal response
- Explain the process on how a MoAb is created from a mouse host and associate correct terminology
- Lymphocytes response
- Fusing lymphocytes with myeloma cells
- Polyethylene glycol
- HAT medium
- Cloning and separating
- Finding the right clone
- Propagation
- Types of immunoglobulins (IgG)
- Whole IgG structure
- Fragmented IgG structure
- Pepsin
- Papain
- F(ab')2, Fab, Fc
- Fragmented vs whole MoAbs?
- Apply the correct radionuclide to the type of MoAb being used
- Consider the type of radiation being used: beta vs. gamma
- Diagnostic vs. therapeutic
- Time between injection and scan
- HAMA response
- Bi-functional chelator
- What is the difference between affinity and avidity
- Strength of attraction
- Integrity of the bond - consider gamma vs beta
- What is the difference between immunoreactive fraction and immunospecificity?
- Free MoAb
- Loss of specificity of the bond
- Compare whole IgG OnocScint to fragmented IgG Arcitumomab. Both are used to diagnose colon cancer.
- Related tumor imaging to lab values
- Gleason score/sum - link
- PSA levels, density, TRUS, and velocity - link
- What does it mean when you say the tumor cell is well vs poorly differentiated - link
- Prostate Cancer - link
- Evaluate non-nuclear procedures used to assess this disease
- DOTATATE - link
- What is the difference between the different DOTAs
- Compare DOTA to Octreotide
- 177Lu-DOTA - link
- Eight week time lines
- What happens during the day of therapeutic administration
- Use of antiemetic
- Use of amino acids
- Pertechnetate physiology -Link
- Compare the relationship between interstitial fluid and the vascular pool
- Assess how the human system picks up and/or eliminates pertechnetate in the following organs
- Kidney
- Stomach and GI track
- Thyroid
- Brain
- Choroid Plexus
- Sweat glands
- Consider the following scenarios
- How would Lugol's affect pertechnetate uptake
- Questionable results from an In Vivo RBC tag
- Not administering KClO4- prior to a pertechnetate brain scan
- Looking at a bone scan when is there too much free tech?
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11/23