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Identification of regulatory sites in genomic DNA
How to avoid being fooled by imposters?
The Discovery: HetQ - The missing link
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You are studying the regulation of the gene hetR, whose product is known to be critical in controlling heterocyst differentiation. You're focusing on the protein HetQ, which you believe regulates the expression of hetR. In examining the sequence upstream from hetQ, you happen to notice the presence of the sequence:
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atctGTAacatgagaTACacaatagcatttatatttgcttTAgtaTctct
The capital letters, you recognize, meet all the requirements of a binding site for the protein NtcA, known to mediate the expression of many genes sensitive to nitrogen-deprivation. Maybe, just maybe, you have accidently discovered the missing link that connects nitrogen-deprivation to the regulation of heterocyst genes!
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