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This result doesn't make sense! This region of the lef gene is highly conserved. If PCR picked up the fragment, then Blast should pick up the sequence. Maybe you've encountered a novel toxin sufficiently related to lef to permit PCR amplification, but not related enough to match known lef sequences in GenBank. This explanation doesn't sound very compelling, but you can think of no better at the moment, so you translate the DG47 sequence, submit the translated sequence to Blast to search for similar proteins, getting the results displayed by clicking here. |