Reduced Particles MAA Worksheet

Name _____Larry Particular______________

1. Dilute MAA vial with 5 mL of saline. Withdraw a 1 mL aliquot and place it in a 30 mL sterile empty vial.
   Calculate the total # of particles in the 1 mL aliquot:

Total particles in vial = ____4,500,000 (found on label)___


2. Calculate total volume needed to make the kit of reduced particles (all contents go into a 30 mL sterile vial)

3. Calculate the activity of ^99mTc required for patient dose. See dose ranges.
Dose in mCi x total volume (activity x volume (step 2) define total ^99mTc in mCi.
Adult dose is usually 3.5 mCi, however, pediatric dose must be acquired by the physician.
Total activity is then determined below:

4. From the elution vial add the required ^99mTc activity to the 30 mL vial and note its volume.

5. Incubate for 15 minutes. Smaller particle amount may require up to 30 minutes incubation.


6. Calculate the volume of normal saline needed in the 30 mL vial.

7. Complete TLC on the final product.
8. From the above data prove that a 1.5 mCi dose has 75,000 particles.