|
Action
|
Explanation and notes
|
| Go to http://www.ncbi.nlm.nih.gov/ |
This is the National Center for Biological Information
(NCBI) site, home of Blast and much else |
| Click on the Entrez on the
blue bar near the top |
Entrez is a tool to download individual sequences held
in GenBank |
| Click in the Search
box (upper left) and select protein |
GenBank has many types of sequence. For example, it has
the DNA sequence for the fdnG gene, which you don't want. |
| In the for
box (next to the Search box) and type
in fdnG AND Escherichia,
and click the GO
button. |
There are many ways you can find the protein you want.
For example, you could specify formate dehydrogenase
AND Escherichia[ORGN] specifying the Escherichia appears in the
Organism field. AND is optional, but if you
put it in, it has to be in capital letters. |
| Scan down until you see NP_415991.
Click on it. |
You should see the GenBank entry for the protein. It
begins LOCUS NP_415991 |
| Download the sequence to whatever directory you keep
Perl programs in. |
Use your browser File SaveAs facility, however it does
it. |
| Congratulate self |
You're done! |