Bierle CJ, Anderholm KM, Wang JB, Mcvoy MA, Schleiss MR
Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/
Cas9-Mediated Gene Editing
J Virol 90:6989 –6998
Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/ Cas9-Mediated Gene Editing
J Virol 90:6989 –6998
(Translated by Mario Melchor-Guerra)
Experiment: Epitope tagging of GP133 by homology-directed repair
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Homology Directed repair can repair breaks caused by CRISPR/Cas9 editing. This can only be done if a homologous recombination template (HRT) is available. The HRT is put onto the 3’ side of the GP133 sequence and it is mutated so that Cas9 does not target the sequence but it remains the same otherwise. Between the edited and unedited sequence containing HRT, the edited/HRT sequence shows that foreign DNA can be added to GPCMV by HDR after Cas9 cleaves into the GP133 sequence.
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Table 3
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