Bierle CJ, Anderholm KM, Wang JB, Mcvoy MA, Schleiss MR
Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/
Cas9-Mediated Gene Editing
J Virol 90:6989 –6998
Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/ Cas9-Mediated Gene Editing
J Virol 90:6989 –6998
(Translated by Mario Melchor-Guerra)
Experiment: Deletion of GP133 by CRISPR/Cas9 editing
|
GPL cells were cotransfected with gRNA targeting the 5’ or 3’ side of GP133 and plasmids. After 96 hours of being infected, the virus containing media had the polymerase chain reaction done to them using GP133 primers. Virus containing media with only one gRNA contained about 582 bp while the one with two gRNA contained 278 bp. This shows that CRISPR/Cas9 can be used to delete specific parts of the viral genome.
|
Table 2
|