Bierle CJ, Anderholm KM, Wang JB, Mcvoy MA, Schleiss MR
Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/
Cas9-Mediated Gene Editing
J Virol 90:6989 –6998
Targeted Mutagenesis of Guinea Pig Cytomegalovirus Using CRISPR/ Cas9-Mediated Gene Editing
J Virol 90:6989 –6998
(Translated by Mario Melchor-Guerra)
BACs
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They are done by using E. coli to make mutant viruses. They are very stable and will usually not change over time. BACs allow for even the smallest change to the viral sequence
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