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Brian Magliaro (UR '98) |
Current position
Environmental consultant, URS Greiner, Woodward, Coyde
Project (Fall 1997 - Spring 1998)
Phage T4's Ability to Protect Plasmid
DNA from Restriction by E. Coli
The methylation and glucosylation that occurs during replication of the virus T4 allows its DNA to enter E. coli cells without being destroyed by restriction enzymes. If a DNA replication signal from T4 is added to plasmid DNA, the virus will replicate the plasmid to form long concatamers of methylated and glucosylated DNA. These concatamers, however, failed to withstand restriction by E. coli. Does this mean that T4 possesses some other advantage besides just methylated and glucosylated DNA in order to withstand restriction? We are currently experimenting with the properties of T4 that allow it to withstand the restriction.
Presentations
Magliaro B (1998). Resistance of T4 virus to restriction by E.
coli: Utilization or an evoluationary missing link? Arts and
Sciences Student Symposium. University of Richmond.
Magliaro B (1998). Resistance of T4 virus to restriction by E. coli: Utilization or an evoluationary missing link? National Conference on Undergraduate Research. Salisbury State University.