Lab 1: Serial dilution of a bacterial culture
Problem: Figure out volumes used in mixing two pure cultures
together
Notes
on reports
Sample lab report
Lab 2: Isolation DNA fragment from plasmid DNA for
cloning
Problem: Identify plasmid and figure out how to clone
a given fragment from it
Gels from Lab 2: Section
2
Important
Clue: Supercoiling of DNA within living cells
Notes
on reports
Sample lab report
Lab 3: Genetic analysis of mystery mutant of Drosophila
melanogaster
Problem: Characterize three mutant strains and map the
underlying mutations
PC-Fly:
Instruction Manual
How
to refine map distance calculations
Notes
on Progress Reports
Lab 4: Recombination and complementation to map genes
of bacteriophage T4
Problem: Map two mutations within rII region of
T4
Lab 5: Regulation of the lac operon
Problem: Identify lac mutations in four mystery
strains
Lab 6: The Agrobacterium tumefaciens Genome
Project
Problem: Figure out all you can about a newly sequenced
region from the Agrobacterium genome
Initial
instructions on analyzing mystery sequence
2nd
Annual UR Symposium on Agrobacterium Genomics: Program