BNFO 491 
Molecular Biology Through Discovery
Questionnaire on notes: DNA Structure and Meselson & Stahl (1958)
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WARNING! Please don't use double quoatation marks in your answers.
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Fall 2012 


I. Basic Information

A. Your name 
II. Old business
A. If you have not posted evidence to support an existing annotation of a T4 genes, what's preventing you from doing so?
     

B. If you have posted evidence to support an existing annotation of a T4 gene, are you happy with your evidence?
     

C. Have you tried out the analysis tool for Benzer (1959)?
    If so, was it helpful? Did you find any obstacle in using it?
    Have you been able to transform go from Fig. 4 into Fig. 5?
     

D. Any areas of discomfort in understanding Benzer's article? If so, what?
     

E. Have you contacted a prospective faculty mentor? If so, how did it go? If not, any problem?
     

III. DNA replication, Part 1

A. How far have you gotten in the part 1 of the notes?
     

Consider how comfortable you are with the following topics regarding DNA replication:

B. The need for a pre-existing 3'-OH end, generally an RNA primer

C. How Benzer's discovery that an rII mutant of T4 could not plate on E.coli K12 opened the possibility of determining the internal structure of a gene

D. The concepts of leading and lagging strands

E. The requirement that DNA synthesis always proceeds 5' to 3'

F. Proofreading and accuracy
     

Choose up to two study questions that you would most like discussed in class:

SQ1  SQ2 SQ3 SQ4 SQ5 SQ6 

IV. Meselson and Stahl

A. How far have you gotten in the companion to Meselson and Stahl?
     

Consider how comfortable you are with the following topics regarding Meselson & Stahl:

B. The topological problem implicit in the replication model presented by Watson and Crick

C. Dispersive replication, and how it addresses the topological problem

D. The critical differences between the three featured models of DNA replication

E. How heavy isotopes can distinguish new from old DNA

F. Equilibrium density centrifugation

G. How to read Fig. 4

H. The relationship between Meselson and Stahl's results and the predictions of the three featured replication models

I. How Delbrück's topological problem was ultimately resolved
     

Choose up to six study questions that you would most like discussed in class:

SQ1  SQ2 SQ3 SQ4 SQ5 SQ6  SQ7  SQ8  SQ9 
SQ10 SQ11 SQ12 SQ13  SQ14 SQ15 SQ16 SQ17 
SQ18  SQ19 
V. Miscellaneous

Any other comments, questions, suggestions, or concerns you may have?

Thanks!

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