BNFO 491 
Molecular Biology Through Discovery
Questionnaire on notes: DNA Structure and Benzer (1959)
(please press SUBMIT button when finished)
WARNING! Please don't use double quoatation marks in your answers.
(The questionnaire program dies when it encounters them!)
Fall 2012 


I. Basic Information

A. Your name 
II. Old business
A. Do you feel comfortable now in finding and posting evidence to support existing annotations of T4 genes?
     

B. Do you feel comfortable now in deriving the sequence of insulin directly from the result tables of Sanger and Tuppy (1951)?
    Are you confident that your chain of reasoning from spots through combination of spots to polypeptide sequence is valid?
     

III. DNA Structure
(No need to respond to this section if you've done so in an earlier questionnaire and nothing's changed)

A. How far have you gotten in DNA Structure?
     

B. Have you looked over web sites that are designed to help you understand x-ray diffraction?
    Do you feel comfortable in deriving from Franklin & Gosling's photograph the helical structure of DNA?
    Do you see what in the picture specifies the internucleotide distance?
     

C. Have you carefully examined Fig. 5 in the DNA structure notes (Chargaff's results)?
    Are you comfortable interpretting the table?
     

D. Have you tried out the Build Your Own DNA exercise?
    Do you feel comfortable using the nucleotide models in the exercise to obtain a structurally reasonable DNA double helix?
     

E. Anything further you want to say concerning DNA structure?
     

IV. Benzer (1959)

A. How far have you gotten in the companion to Benzer (1959)?
     

B. Have you tried out the analysis tool? If so, was it helpful? Did you find any obstacle in using it?
    Have you been able to transform go from Fig. 4 into Fig. 5?
     
Consider how comfortable you are with the following topics (as defined by the companion to Sanger and Tuppy):

C. Why determining the structure of genes was an topic that had to be addressed

D. How Benzer's discovery that an rII mutant of T4 could not plate on E.coli K12 opened the possibility of determining the internal structure of a gene

E. What is meant by the topology of a gene

F. What is meant by recombination and how it relates to distance between genetic markers

G. Why some mutants are able to recombine to produce viable progeny on K12 and others cannot

H. What is dictionary order and how it relates to a genetic map

I. What the recombination matrix, Fig. 4, represents

J. How the recombination matrix relates to the genetic map shown in Fig. 5

K. How this analysis relates to the topology of the rII gene
     

Choose up to six study questions that you would most like discussed in class:

SQ1  SQ2 SQ3 SQ4 SQ5 SQ6 SQ7  SQ8  SQ9 
SQ10 SQ11 SQ12 SQ13  SQ14 SQ15 SQ16 SQ17 
SQ18  SQ19 SQ20 SQ21 SQ22 SQ23 SQ24 SQ25 
SQ26 SQ27 SQ28 SQ29 SQ30 SQ31 SQ32  SQ33 
SQ34 SQ35 SQ36 
V. Miscellaneous

Any other comments, questions, suggestions, or concerns you may have?

Thanks!

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