This function (see A) enables you to mutegenize a derivative of
bacteriophage T4 (or the phage itself) with acridine yellow. The
mutagenized phage may be used to infect either E. coli B or K12.
You'll want to use this function to generate suppressor mutations of T4 strain FC0,
for example.
Type in the number box the number of phages to be mutagenized, which depends on whether you are screening for a relatively frequent event or selecting for a relatively rare event. You might well profit from a visit to the page How many phages to use in experiments. After typing the number, press Enter to close the box (it will turn from white to gray).
To select the phage strain to be mutagenized, click the strain entry box (the box will turn white and become highlighted with red dots; see B), and select a strain from the VARIABLES menu (the strains you see may differ from those in the figure shown at the right).
To select the E.coli strain to be infected, mouse over the green arrow Option icon, select either strain, and then click Apply (see C).
When both boxes have been filled in and an E.coli strain has been selected, then execute the function by double clicking MUTAGENIZE
Output
If no plaques are produced by the infection, you'll see a message at the bottom of the screen (see D). Otherwise a popup window will appear listing the phenotypes of all the plaques (see E).
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