BNFO 300 
Molecular Biology Through Discovery
Path to class (Thursday, Nov 5)
Gene Regulation and Bacteriophage
(please press SUBMIT button when finished)
Fall 2019  


My advice on the matters for the day (as listed in the calendar):

  • Gene Regulation: You can breeze through most of the nine pages of notes on this topic without much loss. The one exception is Fig. 6. If you come to an understanding of that figure, you've gained most of what the notes have to offer. I suggest that you put most of your effort into Fig. 6 and the ~2 pages discussing that figure.
     
  • Problem Set 7: Question 5 of Problem Set 7 is intended to help you understand Fig. 6 of the notes by giving you the opportunity to put your fingers on the relevant sequences. You might also take a look at Question 1 (be sure that you are looking at the 2019 version of the problem set).
     


I. Basic Information

A. Your name  
II. Old business (no need to repeat anything you've said in a previous questionnaire)
  1. Brenner et al (1961) - experiment and results
    Regarding Brenner et al (1961) and its companion, given your new insight into the experiments, can you understand the results found in the different figures and draw conclusions from them?

     
  2. Problem Set 7
    Any issues answering Question 3 in Problem Set 7 (redrawing figures from Brenner et al (1961)?

     
  3. Research proposal
    Do you have an idea on how to go about preparing an outline of your developing research proposal?
III. Gene regulation

A. How far have you gotten in the notes entitled Gene Regulation and Bacteriophage?
     

B. Consider how comfortable you are with the following and comment below as to how class might be most useful to you:

  • Do you understand the different levels of gene regulation?
  • Do you understand the relationship between transcriptional proteins (like RNA polymerase and repressors) and specific DNA sequences (like promoters and operators)?
  • Do you recognize common features of the sequences of actual promoters and operators in the cI-cro region of lambda?
  • Do you understand how the interplay between the lambda repressor and Cro protein might achieve a stable decision of lysogeny vs lysis?
  • Do you understand the power of regulatory feedback loops?
  • Any other regulation-related issues?
     
IV. III. Miscellaneous
Any other comments, questions, suggestions, or concerns you may have?
(nb. Issues concerning your proposal are always welcome)

Thanks!

REMEMBER TO CLICK SUBMIT!