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My advice on the matters for the day (as listed in the calendar):
- Brenner et al (1965): This can be a difficult paper to wade through. Use the companion to guide you through the logic of an interesting slice of the article.
- Alien Genetic Code: Try your hand at using Alien Genetic Code simulation simulation to understand the Jones & Nirenberg experiment yourself. Bring technical and conceptual problems to class.
- Problem Set 5: Bring whatever remaining questions you may have regarding any problem in Problem Set 5.
- Problem Set 6: Take a look at it if you like, but not necessary.
- Translation of an article (if you're doing one):
If you haven't already created a website (as described) now would be a good time to do it.
I. Basic Information
A. Your name
II. Old business
- Jones & Nirenberg (1962) Survey of RNA codewords
Last call on Jones & Nirenberg (1962) and its companion Any issues?
- Problem Set 5?
Any specific problems bedeviling you in Problem Set 5? If so, relate what you have done, what obstacle you've encountered, and in what ways you've tried to overcome it.
- Semester Project?
Translators: Were you able to set up a web site on VCU's people server?
Everyone: Progress? Problems?
Summaries: Clear on what to do?
III. Alien Genetic Code
- Have you given Alien Genetic Code a try?
- Encountered any technical difficulties?
- Do you see how the tools you have at your disposal are the same used by Jones and Nirenberg?
- Progress in breaking the code?
IV. Brenner et al (1965) and its companion
A. How far have you gotten in the companion to Brenner et al (1965)?
B. Consider the following:
- Do you recognize the logical segments of the article?
- Do you understand the nature of suppressible mutations and suppressor strains?
- Do you understand how the phage T4 rII system works, permitting the recognition of mutant rII genes and the selection of strains with the wild-type rII phenotype?
- Do you see how a nonsense mutation might lead to chain termination (perhaps using your superior 21st century knowledge)?
- Do you understand how hydroxylamine leads to a particular kind of mutation?
- Do you see how the mutations induced by hydroxylamine might lead to insights as to the sequence of suppressible nonsense mutations?
- Do you understand the experiment that produces set K and set B amber mutants?
- Do you understand the logic that leads to the elucidation of the sequence of the amber codon?
With this in mind, how would class time best be spent to suit your needs?
V. Miscellaneous
Any other comments, questions, suggestions, or concerns you may have?
(nb. Issues concerning your proposal are always welcome)
Thanks!
REMEMBER TO CLICK SUBMIT!
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