BNFO 300 
Molecular Biology Through Discovery
Questionnaire on notes: Hemoglobin and Perutz et al (1965)
(please press SUBMIT button when finished)
Fall 2018  


My advice on the matters for the day (as listed in the calendar):

Protein
  • Install browser that can run the hemoglobin tutorial: See advice for details. In brief:
    • If you will be using a PC, download and install portable Firefox ESR version 52.
      The version of Firefox you already have will almost certainly not work with the tutorial
    • If you will be using a Mac, try Safari (and let me know if it works or not)
     
  • Hemoglobin Tutorial: Main topic for today. Section III of the companion should be of help. Parts 4 and 5 (maybe 6) are the most important parts of the tutorial. Shouldn't be a big time expenditure.
     
  • Problem Set 2, Problem 4: Make some progress with the community approach described in Problem Set 2, problem 4, to reproducing Sanger and Tuppy's work. We'll discuss progress and continue in class.
     
  • Perutz et al (1965): Just make sure you've downloaded the right article, then skim Sections I and II of the companion to the article. Section III is more important, but that's related to the Hemoglobin Tutorial (below).
     
  • Problem Set 3, Problem 2: Probably no time for this (unless you request it).


I. Basic Information

A. Your name 
II. Old business (no need to repeat anything you've said in a previous questionnaire)
  1. Sanger & Tuppy (1953)
    Anything further you'd like to discuss regarding the article Sanger & Tuppy (1953) and its companion?
    Do you understand how to go from observations (raw chromatographic results) to conclusions (sequence of peptides)?
    Were you able to answer Study Question 17 (creating two chromatograms that led to the conclusions listed in Sager & Tuppy's Table 3 Spot 1)?

     
  2. Problem Set 2, Problem 4
    Progress in the community project? Any issues?
III. Perutz et al (1965)
A. Have you found and downloaded the article Perutz et al (1965) cited in the companion?
    Are you sure you got the right one? How do you know?
   

B. How far have you gotten in a productive reading of the article?
    You can answer by referring to the last section you read or the last study question you addressed (e.g. SQ12) in the companion.
   

Consider how comfortable you are with the following topics (as defined by the companion to Perutz et al):

  • The overall questions addressed by the article
  • The connection between Fig. 1 and Table 2
  • Study questions 1 and 2

With this in mind, how could class time best be spent to meet your needs?
As always, refer to specific matters and relate your current understanding and efforts you've made to address your discomfort.
Do you have any other questions concerning the material in the notes?

IV. Hemoglobin Tutorial
A. Were you able to access Hemoglobin Structure?
    Any problems finding an appropriate browser?
   

Consider how comfortable you are with the following topics (as defined by the Hemoglobin tutorial and the companion to Perutz et al):

  • The different ways protein structures are presented graphically, pros and cons
  • The overall structure of hemoglobin
  • The basis for the formation of alpha helices
  • The role of hydrophobicity in the formation of hemoglobin
  • Study questions 1 and 2

With this in mind, how could class time best be spent to meet your needs?
As always, refer to specific matters and relate your current understanding and efforts you've made to address your discomfort.
Do you have any other questions concerning the material in the notes?

V. Miscellaneous
Any other comments, questions, suggestions, or concerns you may have?

Thanks!

REMEMBER TO CLICK SUBMIT!