S. pneumoniae can cause many types of illnesses with many of these illnesses being life-threatening (bloodstream infections, pneumonia, meningitis, ear infections, etc.). Almost a third of the proteins’ functions in S. pneumoniae; however, are unknown.

To find these proteins' function, a yeast-2-hybrid approach would be the traditional approach. A yeast-2-hybrid approach, unfortunately, often does not lead to the characterization of many of these unknown proteins. In order to characterize a majority of these unknown proteins' functions, another method should be used on top of the yeast-2-hybrid to produce the highest percentage of characterization as possible. Wuchty et al. (2017) utilize a bacterial meta-interactome with other well-studied bacteria to characterize these unknown protein functions.

• Abstract
• Introduction
• Experiments
  • Formation of Bacterial Meta-Interactome
  • Meta-Interactome in Determining Protein Function
  • Quality Control Check of Meta-Interactome against Yeast-2-Hybrid
  • Function Prediction of S.pneumoniae proteins
• Implications
  • Future of Meta-Interactomes in Protein Function Prediction
• Support pages
  • Yeast-2-Hybrid Approach
  • Ortholog Determination
  • ROC Curves