1. Brenner et al (1961) - the rationale and the experiment
   Regarding Brenner et al (1961) and its companion, do you understand the three hypotheses they were trying to test?
   Any concerns about Problem Set 7, question 3 (redrawing figures from Brenner et al (1961))?
   
    
2. Gene regulation
   Any concerns about the notes on Gene Regulation and Bacteriophage, particularly:
   • The different levels of gene regulation?
   • The relationship between transcriptional proteins (like RNA polymerase and repressors) and specific DNA sequences (like promoters and operators)?
   • Common features of the sequences of actual promoters and operators in the cI-cro region of lambda?
   • The power of regulatory feedback loops?
   • How the interplay between the lambda repressor and Cro protein might achieve a stable decision of lysogeny vs lysis?
   • Problem Set 7, question 5 (finding regulatory motifs in a phage that isn't lambda)?
   • Any other regulation-related issues?
   
   
    
3. Research proposal?
   Are you on track with a summary that tells a full story about a key published experiment?
   Do you have an idea on how to go about preparing an outline of your developing research proposal?
   Any other issues concerning your proposal?