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My advice on the matters for the day (as listed in the calendar):
Protein
- Sanger & Tuppy (1951): Main topic for today. Go through the Results section, with the companion to the article by your side. Don't leave class without understanding how the experiment produces the results shown in Sanger & Tuppy's Figures 5 through 16 and how these results lead to the amino acid sequence of one chain of insulin. If you go as far as you can before class, then class can be used to help you overcome the obstacles you will surely encounter.
- Problem Set 2: Protein: We will spend time on the critical Problem #4 of Problem Set 2in class.
Note: Problem Set 2 was recently changed. Make sure that the date 8 Sep 2017 appears in the upper right corner. If you see an earlier date, then you are looking at a cached copy on your own computer. To remove the cached copy (in Firefox):
- Open up Problem Set 2 (wrong version)
- Click the History tab at the top of the Firefox window, select Show All History, click Today
- Find PS2.pdf (or maybe PS2-1.pdf, PS2-2.pdf, etc)
- Right click the name and click Forget About This Site
- Go back to the link to Problem Set 2 and open it again
Research Proposal:
- Progress reports: By this time you may have focused on a single candidate mentor and may have already made initial contact. Be prepared to give an overview of your candidate (particularly if you did not do so in class September 5), what research problems each faculty candidate addresses and the types of experiments they do. Be prepared also to justify why you think the experiments relate to molecular biology (how does something work at the molecular level).
I. Basic Information
Your name
II. Old business
(no need to repeat anything you've said in a previous questionnaire)
- Research Proposal
Any concerns you'd like to raise concerning your efforts to make progress on your research proposal (particularly in finding a mentor)?
- Sanger & Tuppy (1953) - Sections I to III
Anything further you'd like to discuss regarding the article Sanger & Tuppy (1953) and its
companion (Sections I to III)?
Do you have the article by Sanger & Tuppy in hand? You're sure it's the right one?
Do you understand what alternative models of protein structure Sanger & Tuppy hoped to distinguish?
Do you understand how paper chromatography (see
presentation) can be used to separate peptides and identify amino acid?
Do you understand what the paper chromatogram figures in the article signify and why certain amino acids travel faster in one solvent system than another?
III. Sanger & Tuppy (1951)
- How far have you gotten in a productive reading of the article (not just the companion)?
You can answer by referring to the last section you read or the last study question you addressed (e.g. SQ12) in the companion.
- Consider how comfortable you are with the following topics (as defined by the companion to Sanger and Tuppy):
- The overall strategy Sanger & Tuppy used to determine the sequence of insulin (i.e. Fig. 4 in the companion)
- How the identity of amino acids in chromatogram spots is determined
- The relationship between the figures and tables in Sanger & Tuppy (1951)
- How the order of aminio acids in a peptide is determined
- How to gain information from the tables that can be used to deduce the sequence of insulin
- How to use this information to deduce the sequence of insulin
- Any study question
With this in mind, and any other concerns that arise in your mind, how could class time best be spent to meet your needs?
As always, refer to specific matters and relate your current understanding and efforts you've made to address your discomfort.
IV. Problem Set 2, Problem 4 (community effort)
Have you looked over Problem Set 2, Problem 4 and recognize it as a replay of Sanger & Tuppy (1953)?
Have you looked over the effort to organize the class into groups to answer Problem Set 1, Problem 7?
Do you foresee any problems in accomplishing the task?
V. Miscellaneous
Any other comments, questions, suggestions, or concerns you may have?
Thanks!
REMEMBER TO CLICK SUBMIT!
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