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I. Basic Information
A. Your name
II. Old business
(no need to repeat anything you've said in a previous questionnaire)
A. DNA Replication
Anything further you'd like to discuss regarding
the notes
DNA Replication, Part I?
Why a need for a free 3'-OH end and an RNA primer? Why 5' to 3'?
B. Anything else?
Anything else from any time during the semester you'd like to discuss? (specificity welcome!)
III. Meselson and Stahl
A. How far have you gotten in Meselson and Stahl (1958) and its
companion?
B. Consider how comfortable you are with the following topics regarding Meselson & Stahl:
- The topological problem implicit in the replication model presented by Watson and Crick
- Dispersive replication, and how it addresses the topological problem
- The critical differences between the three featured models of DNA replication
- How heavy isotopes can distinguish new from old DNA
- What properties of cesium chloride are helpful in this procedure
- Equilibrium density centrifugation
- How to read Fig. 4 (e.g. what do position and ultraviolet absorption indicate)
- The relationship between Meselson and Stahl's results and the predictions of the three featured replication models
- How Delbrück's topological problem was ultimately resolved
Please relate your thoughts, but no need to repeat anything you've previously submitted
IV. Miscellaneous
Any other comments, questions, suggestions, or concerns you may have?
Thanks!
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