I. Basic Information
A. Your name
II. Old business
(no need to repeat anything you've said in a previous questionnaire)
- Problem Set 3
Anything further you'd like to discuss regarding
Problem Set 3?
- DNA Structure
Anything further you'd like to discuss regarding
the notes
DNA Structure and
the notes on diffraction?
How the helical structure of DNA and internucleotide distances
can be discerned from Franklin and Gosling's x-ray photograph?
The nature and biological roles for palindromic sequences?
- How to read a research article
Anything further you'd like to discuss regarding
the mini-article
(describing an experiment concerning DNA replication) and the
suggestions how to go about
reading it (and any research article)?
III. DNA replication
A. How far have you gotten in the part 1 of the notes entitled
DNA Replication, Part I?
B. Consider how comfortable you are with the following topics regarding DNA replication:
- The need for a pre-existing 3'-OH end, generally an RNA primer
- The concepts of leading and lagging strands
- The requirement that DNA synthesis always proceeds 5' to 3'
- Proofreading and accuracy
IV. Meselson and Stahl
A. How far have you gotten in Meselson and Stahl (1958) and its
companion?
B. Consider how comfortable you are with the following topics regarding Meselson & Stahl:
- The topological problem implicit in the replication model presented by Watson and Crick
- Dispersive replication, and how it addresses the topological problem
- The critical differences between the three featured models of DNA replication
- How heavy isotopes can distinguish new from old DNA
- What properties of cesium chloride are helpful in this procedure
- Equilibrium density centrifugation
- How to read Fig. 4 (e.g. what do position and ultraviolet absorption indicate)
- The relationship between Meselson and Stahl's results and the predictions of the three featured replication models
- How Delbrück's topological problem was ultimately resolved
Please relate your thoughts
V. Miscellaneous
Any other comments, questions, suggestions, or concerns you may have?
Thanks!
REMEMBER TO CLICK SUBMIT!
|