BNFO 300 
Molecular Biology Through Discovery
Questionnaire on: DNA Replication and Meselson & Stahl (1958)
(please press SUBMIT button when finished)
Fall 2014  


I. Basic Information

A. Your name 
II. Old business (no need to repeat anything you've said in a previous questionnaire)
  1. DNA Structure
    Any parting thoughts on the notes for DNA Structure?
    In particular, are you happy with your understanding of diffraction experiments?
    Did the presentation on the subject help? Do you need anything else?

     
  2. Problem Set 6
    Any parting thoughts regarding Problem Set 6 (DNA Replication)?

     
  3. Bibliography
    Any issues in your efforts to find a focused topic for your research proposal and to develop an exhaustive bibliography on that topic?

III. DNA replication

A. How far have you gotten in the part 1 of the notes entitled DNA Replication, Part I?
     

Consider how comfortable you are with the following topics regarding DNA replication:

B. The need for a pre-existing 3'-OH end, generally an RNA primer

C. The concepts of leading and lagging strands

D. The requirement that DNA synthesis always proceeds 5' to 3'

E. Proofreading and accuracy
     

Choose up to two study questions that you would most like discussed in class:

SQ1  SQ2 SQ3 SQ4 SQ5 SQ6 

IV. Meselson and Stahl

A. How far have you gotten in Meselson and Stahl (1958) and its companion?
     

Consider how comfortable you are with the following topics regarding Meselson & Stahl:

B. The topological problem implicit in the replication model presented by Watson and Crick

C. Dispersive replication, and how it addresses the topological problem

D. The critical differences between the three featured models of DNA replication

E. How heavy isotopes can distinguish new from old DNA

F. What properties of cesium chloride are helpful in this procedure

G. Equilibrium density centrifugation

H. How to read Fig. 4 (e.g. what do position and ultraviolet absorption indicate)

I. The relationship between Meselson and Stahl's results and the predictions of the three featured replication models

J. How Delbrück's topological problem was ultimately resolved

      Please relate your thoughts (not just letters), but no need to repeat anything you've previously submitted
     

Choose up to six study questions that you would most like discussed in class:

SQ1  SQ2 SQ3 SQ4 SQ5 SQ6  SQ7  SQ8  SQ9 
SQ10 SQ11 SQ12 SQ13  SQ14 SQ15 SQ16 SQ17 
SQ18  SQ19 
V. Miscellaneous

Any other comments, questions, suggestions, or concerns you may have?

Thanks!

REMEMBER TO CLICK SUBMIT!