Biol 591 
Introduction to Bioinformatics
Fall 2003 

Scenario 3: Comparison of protein sequence against database
Comparison of DG47 DNA sequence with bona fide lef gene


DG47 sequence        1  AATATTGACGCTTTACTACATCAGTCCATCGGAAGTACGTTGTATAATAAAATATATCTG
                        |||||||| |||||| ||||||| ||||| |||||||| ||||| |||||||| ||| ||
M29081 (lef gene) 1831  AATATTGATGCTTTATTACATCAATCCATTGGAAGTACCTTGTACAATAAAATTTATTTG

 
DG47 sequence       61  TATGAAAACATGAATATAAATAACTTAACAGCAACGTTAGGTGCCGATTTAGTAGATTCC
                        |||||||| |||||||| |||||| | ||||||||  ||||||| |||||||| ||||||
M29081 (lef gene) 1891  TATGAAAATATGAATATCAATAACCTTACAGCAACCCTAGGTGCGGATTTAGTTGATTCC

 
DG47 sequence      121  ACAGATAATACAAAAATTAATCGAGGTATATTCAATGAGTTCAAAAAAAATTTCAAATAC
                        || |||||||| ||||||||| ||||||| |||||||| |||||||||||||||||||||
M29081 (lef gene) 1951  ACTGATAATACTAAAATTAATAGAGGTATTTTCAATGAATTCAAAAAAAATTTCAAATAT

 
DG47 sequence      181  AGTATTTCTA
                        ||||||||||
M29081 (lef gene) 2011  AGTATTTCTA

169 out of 190 (89%) nucleotides identical